Effect of basic fibroblast growth factor treatment ...
1. Stroke. 2003 Nov;34(11):2722-8. Epub 2003 Oct 23.
Effect of basic fibroblast growth factor treatment on brain progenitor cells
after permanent focal ischemia in rats.
Wada K, Sugimori H, Bhide PG, Moskowitz MA, Finklestein SP.
Department of Neurology, Massachusetts General Hospital, Harvard Medical School,
Boston, Mass, USA.
BACKGROUND AND PURPOSE: Intracisternal basic fibroblast growth factor (bFGF)
enhances sensorimotor recovery after focal cerebral infarction in rats. One
possible mechanism is stimulation of endogenous progenitor cells in brain. We
investigated the effects of intracisternal bFGF on brain progenitor cells after
METHODS: Proliferating brain cells were labeled with bromodeoxyuridine (BrdU)
before middle cerebral artery (MCA) occlusion or sham surgery in rats. bFGF (0.5
microg) or vehicle was administered intracisternally at 24 and 48 hours after MCA
occlusion, and rats were killed at 7, 14, or 21 days after stroke.
Immunohistochemistry for BrdU and neuron- or astrocyte-specific markers was used
to characterize progenitor cells and their progeny in the subventricular zone and
dentate gyrus of the hippocampus.
RESULTS: Infarct size did not differ among rats with or without bFGF treatment.
MCA occlusion alone increased the number of BrdU-labeled cells in the ipsilateral
subventricular zone at days 7 to 21, and there was a trend toward increased cell
proliferation with bFGF treatment. In the dentate gyrus, the number of
BrdU-labeled cells was increased bilaterally after MCA occlusion (peak at day 7).
This increase was greater after bFGF treatment. In the subventricular zone, 30%
of BrdU-labeled cells were immunopositive for the immature neuron-specific marker
doublecortin at day 7, and their number declined to 2% at day 21. In the dentate
gyrus, the majority of BrdU-labeled cells colabeled with doublecortin at day 7,
becoming NeuN positive at day 21.
CONCLUSIONS: Stroke produces significant changes in progenitor cells in brain
that are augmented by bFGF treatment.
PMID: 14576381 [PubMed - indexed for MEDLINE]